Related Experiment Video

Updated: Aug 16, 2026

RNA-Seq Analysis of Differential Gene Expression in Electroporated Chick Embryonic Spinal Cord
11:13

RNA-Seq Analysis of Differential Gene Expression in Electroporated Chick Embryonic Spinal Cord

Published on: November 1, 2014

Sequence of a chicken erythroblast mono(ADP-ribosyl)transferase-encoding gene and its upstream region

T Davis1, S Shall

  • 1Cell and Molecular Biology Laboratory, University of Sussex, Brighton, UK.

Gene
|October 27, 1995
PubMed

Insights

Researchers cloned the chicken MADPRT gene, encoding a mono(ADP-ribosyl)transferase enzyme. This enzyme shows significant homology to related proteins, suggesting a conserved function in various species.

Area of Science:

  • Molecular Biology
  • Enzymology
  • Genetics

Background:

  • Mono(ADP-ribosyl)transferases (MADPRTs) are enzymes involved in post-translational modification.
  • Understanding the diversity and function of MADPRTs across species is crucial for biological research.

Purpose of the Study:

  • To clone and characterize the MADPRT gene from chicken erythroblasts.
  • To investigate the homology and conserved regions of the encoded protein.

Main Methods:

  • Gene cloning techniques were employed to isolate the MADPRT gene.
  • Bioinformatic analysis was used to compare the chicken MADPRT sequence with known homologs.

Main Results:

  • The chicken MADPRT gene was successfully cloned, encoding a 300-amino-acid protein.
  • The protein demonstrated significant sequence homology (50-52% identity) to rabbit, human, and chicken MADPRTs, particularly in the active site.
  • Analysis of the upstream region revealed characteristics of a promoter sequence.

Conclusions:

  • The cloned chicken MADPRT gene provides a new tool for studying mono(ADP-ribosyl)transferase function.
  • Conserved active site regions suggest a conserved enzymatic mechanism across species.
  • The identified promoter elements offer insights into the gene's transcriptional regulation.

More Related Videos

Efficient Gene Transfer in Chick Retinas for Primary Cell Culture Studies: An Ex-ovo Electroporation Approach08:59

Efficient Gene Transfer in Chick Retinas for Primary Cell Culture Studies: An Ex-ovo Electroporation Approach

Published on: November 2, 2015

Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay08:00

Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay

Published on: June 16, 2021

Related Concept Videos

The Eukaryotic Promoter Region02:40

The Eukaryotic Promoter Region

The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences.  The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...
Leaky Scanning02:28

Leaky Scanning

During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R stands for...
Exon Recombination02:32

Exon Recombination

The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Bacterial Transcription01:53

Bacterial Transcription

RNA polymerase (RNAP) carries out DNA-dependent RNA synthesis in both bacteria and eukaryotes. Bacteria do not have a membrane-bound nucleus. So, transcription and translation occur simultaneously, on the same DNA template.
Transcription can be divided into three main stages, each involving distinct DNA sequences to guide the polymerase. These are: