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Host restriction phenotypes of Salmonella typhi and Salmonella gallinarum
L Pascopella1, B Raupach, N Ghori
1Rocky Mountain Laboratories, Hamilton, Montana 59840, USA.
Abstract:
Salmonella typhi and Salmonella gallinarum phenotypes correlated with mouse host restriction have been identified by using in vitro and in vivo systems. S. typhi is capable of entering the murine intestinal epithelium via M cells, as is Salmonella typhimurium, which causes systemic infection in the mouse. But, unlike S. typhimurium, S. typhi does not destroy the epithelium and is cleared from the Peyer's patches soon after M-cell entry. S. gallinarum appears to be incapable of entering the murine Peyer's patch epithelium. Our in vitro evidence suggests that S. gallinarum is taken up in murine phagocytic cells by a mechanism different from that of S. typhimurium. S. typhimurium is taken up at a higher frequency and is maintained at higher viable counts throughout a 24-h time course in a murine macrophage-like cell line than are S. gallinarum and S. typhi.
Insights
Salmonella typhi and Salmonella gallinarum exhibit distinct host interactions in mice. Understanding these differences in Salmonella pathogenesis is crucial for developing effective treatments.
Area of Science:
- Microbiology
- Immunology
- Pathogenesis
Background:
- Salmonella typhi and Salmonella gallinarum are important bacterial pathogens.
- Understanding host-pathogen interactions is key to controlling Salmonella infections.
Purpose of the Study:
- To identify phenotypes of Salmonella typhi and Salmonella gallinarum that correlate with mouse host restriction.
- To elucidate the mechanisms of entry and survival of these Salmonella serovars in murine models.
Main Methods:
- In vitro and in vivo experimental systems were utilized.
- Murine intestinal epithelium entry via M cells was assessed.
- Phagocytic cell uptake and survival assays were performed using a murine macrophage-like cell line.
Main Results:
- Salmonella typhi enters murine M cells but does not destroy the epithelium and is cleared.
- Salmonella gallinarum appears unable to enter the murine Peyer's patch epithelium.
- Salmonella typhimurium is taken up more frequently and maintained at higher viable counts in murine macrophages compared to S. typhi and S. gallinarum.
Conclusions:
- Salmonella typhi and Salmonella gallinarum exhibit differential interactions with the murine host.
- Distinct mechanisms govern the uptake and survival of Salmonella serovars in phagocytic cells.
- These findings provide insights into Salmonella host restriction and pathogenesis.