Adherence to and invasion of tissue culture cells by Vibrio hollisae

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M D Miliotis1, B D Tall, R T Gray

  • 1Division of Virulence Assessment, U.S. Food and Drug Administration, Washington, D.C. 20204, USA.

Infection and Immunity
|December 1, 1995
PubMed

Insights

Vibrio hollisae invades epithelial cells, multiplying within them. This invasion process involves actin, cellular structures, and receptor-mediated endocytosis, highlighting bacterial pathogenesis mechanisms.

Area of Science:

  • Microbiology
  • Cell Biology
  • Bacterial Pathogenesis

Background:

  • Vibrio hollisae is a bacterial species with implications for human health.
  • Understanding bacterial invasion mechanisms is crucial for developing effective treatments.

Purpose of the Study:

  • To investigate the adherence and invasion of cultured epithelial cells by Vibrio hollisae.
  • To elucidate the cellular processes involved in Vibrio hollisae internalization and intracellular multiplication.

Main Methods:

  • Quantitative studies to assess bacterial adherence and invasion.
  • Microscopy techniques including light, fluorescent, and electron microscopy.
  • Inhibitor studies to determine the cellular pathways involved.

Main Results:

  • Condensed actin was observed around adherent and intracellular Vibrio hollisae.
  • Vibrio hollisae demonstrated intracellular multiplication within epithelial cells.
  • Internalization was identified as a complex process involving protein synthesis, cytoskeletal elements (microfilaments, microtubules), and receptor-mediated endocytosis.

Conclusions:

  • Vibrio hollisae actively invades and replicates within epithelial cells.
  • The invasion mechanism is multifaceted, engaging host cell machinery.
  • These findings provide insights into the pathogenesis of Vibrio hollisae infections.

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