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Oogenesis02:07

Oogenesis

In human women, oogenesis produces one mature egg cell or ovum for every precursor cell that enters meiosis. This process differs in two unique ways from the equivalent procedure of spermatogenesis in males. First, meiotic divisions during oogenesis are asymmetric, meaning that a large oocyte (containing most of the cytoplasm) and minor polar body are produced as a result of meiosis I, and again following meiosis II. Since only oocytes will go on to form embryos if fertilized, this unequal...

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Related Experiment Video

Updated: Jul 11, 2026

Heterotypic Three-dimensional In Vitro Modeling of Stromal-Epithelial Interactions During Ovarian Cancer Initiation and Progression
12:42

Heterotypic Three-dimensional In Vitro Modeling of Stromal-Epithelial Interactions During Ovarian Cancer Initiation and Progression

Published on: August 28, 2012

Trypsinogen expression in human ovarian carcinomas

F Hirahara1, Y Miyagi, E Miyagi

  • 1Department of Obstetrics and Gynecology, Yokohama City University School of Medicine, Japan.

International Journal of Cancer
|October 9, 1995
PubMed
Summary
This summary is machine-generated.

This study investigated trypsin, a digestive enzyme, in ovarian cancer. Trypsinogen was found in ovarian carcinomas, suggesting its role in cancer invasion and metastasis.

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Area of Science:

  • Biochemistry
  • Oncology
  • Molecular Biology

Background:

  • Matrix metalloproteinases and serine proteinases are linked to cancer invasion and metastasis.
  • Trypsin, a serine proteinase, is a digestive enzyme whose role in carcinoma metastasis is explored.

Purpose of the Study:

  • To investigate the implication of trypsin in the invasion and metastasis of human carcinomas.
  • To analyze trypsinogen expression in ovarian carcinoma cell lines and tissues.

Main Methods:

  • Northern blot, RT-PCR, Western blot, and immunohistochemical studies were used.
  • Detection and analysis of trypsinogen expression in ovarian carcinoma cell lines and tissues.
  • Utilized DNA probe for trypsinogen I and antibodies against human trypsin I.

Main Results:

  • Trypsinogen mRNA and protein were detected in ovarian carcinoma cell lines, with high levels in MCAS cell line.
  • All advanced ovarian cancer tissues showed trypsinogen gene expression, particularly serous cystadenocarcinomas.
  • Immunohistochemical staining confirmed trypsin presence in ovarian carcinoma tissues, absent in normal ovaries.

Conclusions:

  • Demonstrates extra-pancreatic production and distribution of trypsinogen in human ovarian carcinomas.
  • Suggests a potential role for trypsinogen in ovarian cancer progression and metastasis.