Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Stereostructure of perimycin A

J Pawlak1, P Sowiński, E Borowski

  • 1Department of Pharmaceutical Technology and Biochemistry, Technical University of Gdańsk, Poland.

The Journal of Antibiotics
|September 1, 1995
PubMed
Summary

The chemical structure of perimycin A was revised, moving a keto group to a new position. Nuclear Magnetic Resonance (NMR) studies confirmed the molecule's stereostructure.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Microbiological studies of the round goby (<i>Neogobius melanostomus</i>) from the Puck Bay, southern Baltic Sea.

Polish journal of veterinary sciences·2023
Same author

Saduria entomon infected with Hysterothylacium aduncum found in situ in the stomach of cod (Gadus morhua) from the Baltic Sea.

Journal of helminthology·2017
Same author

Global analysis of gene expression in maize leaves treated with low temperature. II. Combined effect of severe cold (8 °C) and circadian rhythm.

Plant molecular biology·2017
Same author

Postantibiotic effect and postantibiotic sub-MIC effect of LTX-109 and mupirocin on Staphylococcus aureus blood isolates.

Letters in applied microbiology·2017
Same author

Personality traits as an endophenotype in genetic studies on suicidality in bipolar disorder.

Acta neuropsychiatrica·2016
Same author

Antiviral and lung protective activity of a novel respiratory syncytial virus fusion inhibitor in a mouse model.

The European respiratory journal·2010

Area of Science:

  • Biochemistry
  • Organic Chemistry
  • Spectroscopy

Background:

  • Perimycin A is an antifungal antibiotic belonging to the polyene class.
  • Understanding the precise chemical structure of perimycin A is crucial for its mechanism of action studies.
  • Previous structural assignments for perimycin A required further clarification.

Purpose of the Study:

  • To revise and confirm the gross structure of perimycin A.
  • To elucidate the stereostructure of perimycin A.
  • To provide a definitive structural basis for perimycin A research.

Main Methods:

  • Re-evaluation of spectroscopic data.
  • Nuclear Magnetic Resonance (NMR) spectroscopy, including 1D and 2D techniques.
  • Chemical shift analysis and coupling constant interpretation.

Main Results:

  • The position of the keto group in perimycin A was revised from C-13 to C-5.
  • The stereochemistry of perimycin A was definitively established using NMR data.
  • Comprehensive structural data provides a foundation for future studies.

Conclusions:

  • The revised structure of perimycin A, with the keto group at C-5, is now established.
  • NMR spectroscopy is a powerful tool for determining the stereostructure of complex natural products.
  • This structural elucidation will facilitate further investigations into perimycin A's biological activity and interactions.

Related Experiment Videos