Related Experiment Videos
Response of Saccharomyces cerevisiae strains to antineoplastic agents
A Delitheos1, I Karavokyros, E Tiligada
1Department of Experimental Pharmacology, Athens University School of Medicine, Greece.
Abstract:
The effect of several antineoplastic agents on Saccharomyces cerevisiae strains has been investigated. Minimum inhibitory concentration (MIC), minimum cytotoxic concentration (MCC) and median effective concentration (EC50) were determined to identify strains with inherent sensitivity to the agents tested. Several strains proved to be sensitive to the antimetabolites 5-fluorouracil and methotrexate as well as to doxorubicin and cis-platine. On the contrary m-amsacrine, procarbazine, vinca alcaloids, melphalan and hydroxyurea were inactive at concentrations up to 400 micrograms ml-1. The strain ATCC 2366, the most relatively sensitive to the agents tested, was used for studying the effect of treatment duration and of drug concentration on cell survival. Methotrexate and cis-platine, which according to MIC and MCC tests seemed ineffective for this strain, reduced survival significantly after 6 h of treatment. A correlation of the shape of the survival curves with MIC and MCC values was attempted.
Insights
This study investigated antineoplastic agents
Area of Science:
- * Pharmacology and Microbiology
- * Anticancer drug research
Background:
- * Antineoplastic agents are crucial in cancer therapy.
- * Understanding their effects on non-target organisms like Saccharomyces cerevisiae can offer insights into drug mechanisms and resistance.
Purpose of the Study:
- * To evaluate the sensitivity of Saccharomyces cerevisiae strains to various antineoplastic agents.
- * To determine the impact of treatment duration and drug concentration on yeast cell survival.
Main Methods:
- * Determination of Minimum Inhibitory Concentration (MIC), Minimum Cytotoxic Concentration (MCC), and Median Effective Concentration (EC50).
- * Cell survival assays were performed on the most sensitive yeast strain (ATCC 2366) using varying drug concentrations and treatment durations.
- * Correlation analysis between survival curve shapes and MIC/MCC values.
Main Results:
- * Certain yeast strains exhibited sensitivity to 5-fluorouracil, methotrexate, doxorubicin, and cisplatin.
- * Other agents like m-amsacrine and vinca alkaloids were inactive at tested concentrations.
- * Methotrexate and cisplatin significantly reduced cell survival after 6-hour exposure, despite initial MIC/MCC tests suggesting ineffectiveness.
Conclusions:
- * Saccharomyces cerevisiae strains display differential sensitivity to antineoplastic drugs.
- * Treatment duration is a critical factor influencing drug efficacy, even for agents initially appearing ineffective based on MIC/MCC.
- * Further investigation into survival curves can refine understanding of drug-chemosensitivity relationships.