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A note on the development of a non-aldehyde fixation technique for examining spores of Bacillus subtilis under the
A G Knott1, A C Hann, A D Russell
1Welsh School of Pharmacy, University of Wales College of Cardiff, UK.
The Journal of Applied Bacteriology
|October 1, 1995
Abstract:
Various techniques were studied for fixing spores of Bacillus subtilis prior to examining them by transmission or scanning electron microscopy. A non-aldehyde technique employing carbodiimide in cacodylate buffer produced excellent results and could be of value in studying the cytological changes produced in spores exposed to inimical treatments.