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Related Experiment Videos

Proteoglycan synthesis by corneal explants from developing embryonic chicken

K Nakazawa1, S Suzuki, K Wada

  • 1Section of Radiochemistry, Faculty of Pharmacy, Meijo University, Nagoya.

Journal of Biochemistry
|April 1, 1995
PubMed
Summary
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Chicken cornea proteoglycan synthesis shifts from chondroitin sulfate/dermatan sulfate proteoglycan to keratan sulfate proteoglycan during embryonic development. This change, particularly increased sulfation of keratan sulfate proteoglycan, correlates with the onset and progression of corneal transparency.

Area of Science:

  • Developmental Biology
  • Biochemistry
  • Ophthalmology

Background:

  • Proteoglycans are crucial components of the corneal extracellular matrix.
  • Changes in proteoglycan composition and sulfation are implicated in tissue development and function.
  • Understanding proteoglycan biosynthesis during corneal development is key to understanding transparency acquisition.

Purpose of the Study:

  • To investigate the developmental changes in proteoglycan biosynthesis in embryonic chicken corneas.
  • To correlate proteoglycan synthesis patterns with the onset of corneal transparency.
  • To analyze the sulfation patterns and medium proteoglycan composition during development.

Main Methods:

  • Cultured embryonic chicken corneas (5-18 days) labeled with [3H]glucosamine and [35S]sulfate.

Related Experiment Videos

  • Analysis of labeled corneal and medium proteoglycans using enzymatic digestion (chondroitinase ABC, keratanase II) and HPLC.
  • Quantification of glycosaminoglycan disaccharides to determine sulfation extents and positions.

    • Main Results:

    • Early synthesis (Day 5) dominated by chondroitin sulfate/dermatan sulfate proteoglycan (CSPG/DSPG).
    • A significant increase in keratan sulfate proteoglycan (KSPG) synthesis observed from Day 7, coinciding with fibroblast invasion.
    • KSPG synthesis proportion increased until Day 14, while CSPG/DSPG decreased; KSPG sulfation and medium KSPG proportion increased with age.

    Conclusions:

    • Developmental shift in proteoglycan synthesis from CSPG/DSPG to KSPG occurs during chicken corneal development.
    • Increased KSPG sulfation and altered CSPG/DSPG proportions correlate with the onset and progression of corneal transparency.
    • Preferential acceleration of KSPG catabolism may occur during late corneal development.