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Quantification of erythrocyte aggregation by blood echogenicity: a preliminary study
S M Razavian1, J Levenson, P Peronneau
1Unité INSERM U28, Hôpital Broussais, Paris, France.
The Journal of Cardiovascular Surgery
|August 1, 1995
Summary
This study introduces high-resolution ultrasonography to measure red blood cell aggregation in flowing blood. This novel method quantifies erythrocyte aggregation using blood echogenicity, offering potential for in vivo clinical applications.
Area of Science:
- Biomedical Engineering
- Hematology
- Medical Physics
Background:
- Erythrocyte aggregation assessment is crucial in clinical research.
- Existing methods primarily focus on in vitro analysis.
- A need exists for in vivo measurement of erythrocyte aggregation in flowing blood.
Purpose of the Study:
- To develop and validate a novel method for quantifying erythrocyte aggregation in flowing blood.
- To utilize high-resolution ultrasonography and blood echogenicity for this purpose.
- To explore the potential for in vivo application.
Main Methods:
- Development of an A-mode ultrasound scanner integrated with signal analysis software.
- Use of a closed measurement chamber with a calibrated blood pump for controlled blood flow.
- Determination of blood echogenicity at various shear rates and under influence of aggregating agents.
Main Results:
- An increase in blood echogenicity was observed at low shear rates.
- Addition of high molecular weight substances like dextran and fibrinogen increased blood echogenicity.
- Changes in red blood cell concentration also affected echogenicity.
Conclusions:
- High-resolution ultrasonography can quantify erythrocyte aggregation in flowing blood.
- This technique shows promise for in vivo measurements, particularly in low-shear venous circulation.
- The method provides a new approach to understanding blood flow dynamics and aggregation.