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Related Experiment Videos

Hypoxia-induced electrical changes in striatal neurons

P Calabresi1, A Pisani, N B Mercuri

  • 1Clinical Neurologica, Dip. Sanità, Università di Roma Tor Vergata, Italy.

Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism
|November 1, 1995
PubMed
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Hypoxia causes reversible or irreversible membrane depolarizations in striatal neurons. These electrical changes during oxygen deprivation are independent of excitatory amino acid release.

Area of Science:

  • Neuroscience
  • Cellular Physiology

Background:

  • Hypoxia, or oxygen deprivation, significantly impacts neuronal function.
  • Striatal neurons are crucial for motor control and are vulnerable to hypoxic insults.

Purpose of the Study:

  • To investigate the direct effects of hypoxia on striatal neuron membrane properties.
  • To elucidate the ionic mechanisms underlying hypoxia-induced electrical changes in these neurons.

Main Methods:

  • Intracellular recordings and voltage-clamp experiments in corticostriatal slice preparations.
  • Pharmacological manipulations including tetrodotoxin, low Ca2+/high Mg2+ solutions, EAA receptor antagonists, low Na+ solutions, and ouabain.

Main Results:

  • Hypoxia induced reversible (2-10 min) and irreversible (12-20 min) membrane depolarizations.

Related Experiment Videos

  • Hypoxia caused an inward current and increased membrane conductance, independent of synaptic transmission or EAA receptors.
  • The inward current was reduced in low Na+ solutions and enhanced by ouabain, suggesting Na+ and the Na+/K+-ATPase pump are involved.

    • Conclusions:

    • Acute hypoxia directly alters striatal neuron membrane properties.
    • Excitatory amino acid release is not required for these acute hypoxia-induced electrical changes.
    • The findings implicate ion transport mechanisms, particularly involving sodium, in neuronal responses to hypoxia.