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Immunohistochemical detection of CD31 antigen in normal and neoplastic canine endothelial cells
L Ferrer1, D Fondevila, R M Rabanal
1Department of Animal Pathology, Veterinary School, Universitat Autònoma de Barcelona, Bellaterra, Spain.
Insights
Immunohistochemical detection of CD31 antigen and von Willebrand
Area of Science:
- Veterinary Pathology
- Immunohistochemistry
- Canine Neoplasms
Background:
- Understanding canine vascular disorders requires reliable diagnostic markers.
- Von Willebrand factor (vWf) and CD31 antigen are markers for endothelial cells.
- Their utility in canine tissues, particularly neoplasms, needs further investigation.
Purpose of the Study:
- To evaluate the immunohistochemical detection of vWf and CD31 in canine normal organs and vascular tumors.
- To assess the diagnostic value of CD31 antigen in canine vascular disorders.
Main Methods:
- Immunohistochemistry was performed on paraffin-embedded, formalin-fixed canine tissue sections.
- Detection of von Willebrand's factor (vWf) and CD31 antigen was analyzed.
- Tissues examined included normal organs and various canine neoplasms.
Main Results:
- CD31 and vWf were detected in endothelial cells of most organs, with exceptions in renal glomeruli (vWf negative).
- All examined haemangiomas (15) were positive for both markers.
- Haemangiosarcomas (15) showed positivity for vWf (11/15) and CD31 (15/15). Other neoplasms were negative for both markers.
Conclusions:
- Immunohistochemical detection of CD31 antigen is valuable for studying canine vascular disorders.
- Both CD31 and vWf are reliable markers for diagnosing canine haemangiomas and haemangiosarcomas.
- CD31 is particularly useful in routinely processed canine tissues.
Abstract:
This report describes the immunohistochemical detection of von Willebrand's factor (vWf) and CD31 antigen in paraffin wax-embedded, formalin-fixed tissue sections of canine normal organs and vascular neoplasms. CD31 antigen and vWf were detected in endothelial cells of all organs examined, except for the endothelia of the renal glomeruli, which were negative for vWf. All haemangiomas examined (15) were positive for both markers. Eleven of 15 haemangiosarcomas were positive for vWf and all 15 expressed the CD31 antigen. All other neoplasms investigated (fibrosarcomas, schwannomas, haemangiopericytomas) were negative for both markers. It is concluded that immunohistochemical detection of CD31 antigen is of value for studying vascular disorders of the dog in routinely processed tissue.