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Related Experiment Videos

Influence of coding-end sequence on coding-end processing in V(D)J recombination

B Nadel1, A J Feeney

  • 1Department of Immunology, Scripps Research Institute, La Jolla, CA 92037, USA.

Journal of Immunology (Baltimore, Md. : 1950)
|November 1, 1995
PubMed
Summary
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The sequence of DNA ends significantly influences immune receptor gene recombination, revealing non-random processing patterns. Specific DNA motifs guide nucleotide deletion and addition, shaping the immune system

Area of Science:

  • Immunology
  • Molecular Biology
  • Genetics

Background:

  • Immune receptor diversity (Immunoglobulin and T-cell receptor repertoires) arises from V(D)J recombination.
  • This process involves combinatorial gene segment assembly and junctional modifications (coding-end processing).
  • Coding-end processing includes nucleotide removal and addition, previously thought to be random.

Purpose of the Study:

  • To investigate the mechanism of coding-end processing in V(D)J recombination.
  • To determine if coding-end processing patterns are random or sequence-specific.
  • To identify sequence features that influence nucleotide modification during recombination.

Main Methods:

  • Construction of a large database of immunoglobulin (Ig) and T-cell receptor (TCR) coding ends from in vivo samples.

Related Experiment Videos

  • Analysis of nucleotide deletion and addition patterns at recombination junctions.
  • Correlation of processing patterns with specific DNA sequence motifs (e.g., A.T and G.C stretches).

    • Main Results:

    • Coding-end processing is not random but specific to each coding end.
    • Specific DNA sequence motifs within coding ends influence processing patterns.
    • A strong correlation exists between A.T-rich sequences, absence of G.C-rich sequences, and increased nucleotide deletion.
    • Hairpin intermediate nicking preferentially occurs in A.T-rich regions with weaker base pairing.

    Conclusions:

    • The DNA sequence of coding ends plays a critical role in the non-random aspects of V(D)J recombination.
    • Sequence-specific processing suggests evolutionary selection of coding-end sequences for repertoire control.
    • These findings provide insights into the regulation of immune receptor gene assembly.