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Immunoassays for detecting cytokines: what are they really measuring?

A R Mire-Sluis1, R Gaines-Das, R Thorpe

  • 1Division of Immunobiology, National institute for Biological Standards and Control, Potters Bar, Herts, UK.

Journal of Immunological Methods
|October 26, 1995
PubMed

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Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.