Oxidative stress in radiation-induced interstitial pneumonitis in the rat

The involvement of free radical metabolism in the pathogenesis of interstitial pneumonitis was investigated in an animal model. Male Wistar rats were irradiated at the thoracic region by gamma-rays from a 60Co source. Histopathological examination confirmed that 50% of the rats developed pneumonitis between 2 and 8 weeks following a single dose of 14 Gy. Parallel biochemical studies in the lung of these rats showed that mitochondria and microsomes had higher levels of lipid peroxidation. In the cytoplasmic fraction of the lung the activities of superoxide dismutase and catalase were markedly reduced in the pneumonitic rat. In lung mitochondria, however, the levels of these two enzymes were not significantly altered. On the contrary, lipid peroxidation and superoxide dismutase, as well as catalase activities in lung tissue in the non-pneumonitic group of the irradiated rat were comparable with that of control animals. The results indicate that free radical-induced oxidative stress following thoracic irradiation may be one of the causative factors in the development of interstitial pneumonitis.