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Related Experiment Videos

Molecular relationships between 21 human rhinovirus serotypes

C Horsnell1, R E Gama, P J Hughes

  • 1Department of Biology, University of Essex, Colchester, UK.

The Journal of General Virology
|October 1, 1995
PubMed
Summary

This study analyzed human rhinovirus (HRV) genomic sequences, revealing two distinct molecular clusters. These clusters correlate with antiviral drug sensitivity, suggesting a fundamental division within HRV serotypes.

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Area of Science:

  • Virology
  • Molecular Biology
  • Genetics

Background:

  • Human rhinoviruses (HRVs) are a major cause of the common cold.
  • Understanding HRV genetic diversity is crucial for developing effective antiviral therapies.
  • Previous studies have explored HRV classification based on various criteria.

Purpose of the Study:

  • To analyze the genetic relatedness of human rhinovirus (HRV) serotypes.
  • To investigate potential correlations between molecular groupings and phenotypic characteristics.
  • To identify fundamental divisions within HRV populations.

Main Methods:

  • Polymerase Chain Reaction (PCR) using consensus primers for genetic analysis.
  • DNA sequencing of a specific genomic region encoding 112 amino acids.

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  • Bioinformatic analysis of 21 available HRV sequences, including 12 newly analyzed.

    • Main Results:

    • The majority of HRV serotypes (18/21) showed at least 70% amino acid identity in the analyzed region.
    • Two distinct molecular clusters were identified: a majority group and a smaller group (HRV-3, -14, -72).
    • Membership in these clusters correlated with sensitivity to antiviral drugs, but not with receptor grouping.

    Conclusions:

    • HRV genetic analysis reveals two major molecular clusters with distinct characteristics.
    • Antiviral drug sensitivity appears to be a key indicator of fundamental HRV divisions.
    • These findings provide insights into HRV evolution and potential therapeutic strategies.