Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Protective immunity to the group A Streptococcus may be only strain specific

S A de Malmanche1, D R Martin

  • 1ESR Health: Communicable Disease Centre, Porirua, New Zealand.

Medical Microbiology and Immunology
|December 1, 1994
PubMed
Summary

Opsonic antibodies, crucial for immunity against Group A Streptococcus, may be strain-specific, not just type-specific. This finding impacts our understanding of protective immunity and vaccine development against M1 strains.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Molecular modeling and simulation studies of SELEX-derived high-affinity DNA aptamers to the Ebola virus nucleoprotein.

Journal of biomolecular structure & dynamics·2024
Same author

Observations about declining fertility in a feline breeding colony.

Theriogenology·2023
Same author

Inhibition of Psychrotrophic Bacteria in Refrigerated Milk by Lactobacilli Isolated from Yogurt <sup>1</sup>.

Journal of food protection·2019
Same author

Single-Organ Patients.

The Physician and sportsmedicine·2017
Same author

Radiology Consultations: How to Get Optimal Results.

The Physician and sportsmedicine·2017
Same author

Reducing Dislocations on the Field.

The Physician and sportsmedicine·2017

Area of Science:

  • Microbiology
  • Immunology
  • Bacteriology

Background:

  • M protein in Group A Streptococcus (GAS) facilitates resistance to phagocytosis.
  • Opsonic antibodies targeting M protein are believed to mediate protective immunity.
  • Recent findings indicate genetic diversity within M-types, suggesting potential strain-specific variations.

Purpose of the Study:

  • To investigate if type-specific M1 antibodies exhibit opsonic activity against all M-type 1 strains, regardless of their genetic (restriction fragment length polymorphism) type.
  • To determine the prevalence and specificity of opsonic antibodies in human sera against M1 strains.

Main Methods:

  • Indirect bactericidal assays were employed to test the opsonic activity of human and rabbit M1 antibodies.
  • Human sera were screened for opsonic antibodies against various M-type 1 strains with differing genetic profiles.

Related Experiment Videos

  • Restriction fragment length polymorphism (RFLP) typing was used to characterize bacterial strains.

    • Main Results:

    • Selective variation in opsonic ability was observed in human sera.
    • Out of 44 randomly selected human sera, 11 (25%) possessed opsonic antibodies.
    • Of the 11 sera with opsonic activity, 6 opsonised all tested M1 strains, while 5 showed selective opsonisation of specific strains.

    Conclusions:

    • Opsonic antibodies against M protein may not be strictly type-specific.
    • Antibody specificity can be strain-specific within a given M-type.
    • These findings suggest a more complex immune response to GAS than previously understood, with implications for vaccine design.