Related Experiment Video
Updated: Oct 15, 2026

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization
Published on: April 3, 2014
The distribution of electron trapping in DNA: one-electron-reduced oligodeoxynucleotides of adenine and thymine
1Department of Biophysics, University of Rochester, New York 14642-8408, USA.
Abstract:
A series of single- and double-stranded oligodeoxynucleotides of adenine and thymine, 8 to 12 nucleotides in length, were one-electron-reduced at 10 K in a > 8 M LiCl/H2O glass. The Q-band electron paramagnetic resonance (EPR) spectra of these radicals show that thymine is the dominant trapping site for mobile electrons in these oligomers. The spectra of the reduced oligomers in the series pd(AnT10-n).pd(A10-nTn) with n = 5-->10 showed a trend which is interpreted as either an increase in the probability of trapping at an adenine base in tracks of adenine > 7 base pairs in length, or the presence of different protonated states of the one-electron-reduced bases due to the adoption of a different conformational state for longer tracks of adenine, or a combination of these two possibilities. Analysis of the trends in the EPR spectra of the radicals as a function of sequence using multicomponent analysis is presented.
More Related Videos
10:59Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair
Published on: May 24, 2017
12:15Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter
Published on: May 29, 2019
Related Concept Videos
The DNA Helix
Proofreading
DNA Base Pairing
Role of Reduced Coenzymes NADH and FADH₂
The DNA Helix
Proofreading
Errors During Replication are Corrected by the DNA Polymerase Enzyme