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Effects of anisodamine against myocardial ischemia-reperfused injuries and antilipid peroxidation
1Department of Pharmacology, Fourth Military Medical University, Xi-an, China.
Aim:
Anisodamine (Ani), an alkaloid first isolated in China. To study the relationship between the protective effects of Ani on myocardial cells of reperfused injuries and the antilipid peroxidation.
Methods:
Coronary ligation for 15-min followed by 10 min reperfusion was performed in anesthetized rats.
Results:
Ani 1, 3, 5 mg.kg-1 i.v. 1 min prior to reperfusion could dose-dependently lower the release of creatine kinase (282 +/- 29, 252 +/- 53, 226 +/- 50), counteract the increase of malondialdehyde content (3.3 +/- 1.3, 3.2 +/- 1.6, 3.1 +/- 1.2) in the reperfused myocardium and preserve the SOD activity (41 +/- 7, 46 +/- 8, 55 +/- 8). Ani completely abolished the drop in the contents of principal unsaturated fatty acids (oleic, linoleic, and arachidonic acids) of the membrane lipids in the reperfused myocardium. SOD 75 U.kg-1 i.v. 1 min prior to reperfusion exerted similar effects like Ani 3 mg.kg-1.
Conclusion:
It is suggested that the antilipid peroxidative effect of Ani may contribute to its protection against reperfusion-induced myocardial injuries.