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Development of rapid mercury assays. Synthesis of sulfur- and mercury-containing conjugates
F Szurdoki1, H Kido, B D Hammock
1Department of Entomology, University of California, Davis 95616, USA.
Bioconjugate Chemistry
|March 1, 1995
Abstract:
We have devised rapid analyses for mercury exploiting the high affinity of dithiocarbamate chelators for the mercuric ion. Our first assay is based on a sandwich chelate formed by a ligand supported on the well of an ELISA plate, Hg2+ ion of the investigated sample, and another ligand bound to a reporter enzyme. The second assay utilizes competitive binding of the analyte Hg2+ ions versus an organomercury conjugate to a chelating conjugate. Low ppb sensitivity and high selectivity for Hg2+ ions have been achieved in our pilot studies.