A practical method for stereospecific assignments of gamma- and delta-methylene hydrogens via estimation of vicinal...

Stereospecific assignments are made for gamma- and delta-methylene hydrogens in a protein by means of estimation of vicinal 1H-1H spin-spin coupling constants from a short-mixing-time TOCSY experiment. 3J alpha beta coupling constants, as measured from a P.E. COSY map, are shown to be well correlated with alpha-beta cross-peak intensities of a short-mixing-time (10 ms) TOCSY map. The procedure is illustrated by application to a trypsin-inhibitor protein (M(r) approximately 7 Kd). Thus, gamma-methylene hydrogens of isoleucine residues have been stereospecifically assigned on the basis of 3J beta gamma 1H-1H coupling patterns and intraresidue cross-peak intensities in a NOESY map; gamma-hydrogens of other residues, such as lysine and arginine, have been stereospecifically assigned solely on the basis of cross-peak intensity patterns resulting from coupling of two beta-hydrogens to two gamma-hydrogens, and in conjunction with stereospecific assignments of beta-methylene hydrogens. However, intraresidue NOE intensities are needed if one or two pairs of coupling constants cannot be estimated because of cross peaks either overlapping or occurring proximal to the diagonal. The delta-methylene hydrogens have been stereospecifically assigned on the basis of coupling between two gamma-hydrogens and two delta-hydrogens, in combination with stereospecific assignments of gamma-hydrogens. Stereospecific assignments of side chains should contribute to the overall precision and accuracy of NMR-determined three-dimensional solution structures of proteins.