[Experimental studies of glomerular endothelial cell culture and its production of extracellular matrixes]

We successfully cultured human fetal and bovine glomerular endothelial cells by cell cloning and maintained seven passages. The glomerular endothelial cells were identified by indirect immunofluorescence method. We found that the fetal and bovine glomerular endothelial cells could produce factor VIII related antigen, fibronectin, type IV collagen and laminin. The extracellular matrixes in the cultured supernatant were determined at various stages. The results showed that the glomerular endothelial cells could produce extracellular matrixes steadily without any stimulating factor.