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A widely distributed putative mammalian transcriptional regulator containing multiple paired amphipathic helices,

M S Halleck1, S Pownall, K W Harder

  • 1Department of Biochemistry and Molecular Biology, Penn State University, University Park 16802, USA.

Genomics
|March 20, 1995
PubMed

Insights

The mammalian Sin3 gene (mSin3) shows similarity to yeast SIN3 but cannot replace it functionally. Its widespread detection in mouse tissues suggests mSin3 regulates multiple genes, similar to its yeast counterpart.

Area of Science:

  • Molecular Biology
  • Genetics
  • Gene Regulation

Background:

  • The mammalian Sin3 gene (mSin3) possesses four paired amphipathic helix (PAH) motifs.
  • Three PAH motifs and an extended region show significant sequence similarity (59-70%) to the yeast transcriptional regulator, SIN3.

Purpose of the Study:

  • To investigate the functional relationship and tissue distribution of the mammalian Sin3 gene (mSin3).
  • To determine if mSin3 can functionally substitute for yeast SIN3 in complementation assays.

Main Methods:

  • Sequence similarity analysis between mSin3 and yeast SIN3.
  • Complementation assays using mSin3/SIN3 fusion proteins in yeast.
  • Transcript analysis using mouse tissues to determine mSin3 expression levels.

Main Results:

  • mSin3/SIN3 fusion proteins failed to complement the yeast SIN3 deletion.
  • mSin3 transcripts were detected in various mouse tissues, notably high in testis, lung, and thymus.
  • mSin3 maps to human chromosome 15q24.

Conclusions:

  • Despite sequence homology, mSin3 does not functionally replace yeast SIN3.
  • The broad tissue distribution of mSin3 implies a role in regulating multiple genes, analogous to yeast SIN3.

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