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Cytotoxigenicity in Salmonella serovars
P Malik1, V D Sharma, R Chandra
1Department of Veterinary Microbiology, College of Veterinary Sciences, G.B. Pant University of Agriculture & Technology, Pantnagar, India.
Indian Journal of Experimental Biology
|March 1, 1995
Summary
This study investigated Salmonella cytotoxicity in animal food products. Researchers found varying cytotoxic effects across eight Salmonella serovars on MDBK and Vero cells, with enhanced toxin release using specific treatments.
Area of Science:
- Microbiology
- Cell Biology
- Food Safety
Background:
- Salmonella is a significant foodborne pathogen.
- Understanding Salmonella's cytotoxic potential is crucial for food safety.
- This study examines cytotoxicity of various Salmonella serovars from animal-origin foods.
Purpose of the Study:
- To assess the cytotoxic effects of 22 Salmonella strains from eight serovars on MDBK and Vero cell lines.
- To investigate factors that may augment cytotoxin release.
Main Methods:
- Twenty-two Salmonella strains (eight serovars) isolated from animal-origin foods were used.
- Cytotoxicity was tested on MDBK and Vero cell lines.
- The effect of Polymyxin B sulfate, urea, and cell-sonication on cytotoxin release was evaluated.
Main Results:
- All tested Salmonella strains exhibited cytotoxicity against both MDBK and Vero cells.
- Cytotoxic activity varied significantly among the strains.
- A dose-dependent cytotoxic effect was observed.
- Polymyxin B sulfate, urea, and cell-sonication enhanced cytotoxin release.
Conclusions:
- Salmonella strains from animal-origin foods possess cytotoxic properties.
- Cytotoxicity varies among serovars and is dose-dependent.
- Specific treatments can increase cytotoxin release, relevant for detection and understanding pathogenicity.