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Related Experiment Videos

[Molecular epidemiological study on Vibrio cholerae O139]

Y Gyobu1, S Hosorogi, T Shimada

  • 1Toyama Institute of Health.

Kansenshogaku Zasshi. the Journal of the Japanese Association for Infectious Diseases
|May 1, 1995
PubMed
Summary

Pulsed-field gel electrophoresis (PFGE) revealed genetic diversity among Vibrio cholerae O139 strains. Most epidemic strains shared similar genetic patterns, while non-epidemic and CT-negative strains showed distinct variations.

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Area of Science:

  • Microbiology
  • Genetics
  • Epidemiology

Background:

  • Vibrio cholerae O139 is a significant cause of cholera outbreaks.
  • Understanding the genetic relatedness of V. cholerae O139 strains is crucial for tracking disease transmission and evolution.
  • Pulsed-field gel electrophoresis (PFGE) is a valuable tool for analyzing bacterial genomic DNA

Purpose of the Study:

  • To genetically characterize 56 Vibrio cholerae O139 strains using PFGE.
  • To investigate the genetic diversity and relationships among epidemic and non-epidemic strains.
  • To compare the genetic profiles of CT-gene-positive and CT-gene-negative strains.

Main Methods:

  • Genomic DNA extraction from 56 Vibrio cholerae O139 isolates.
  • Digestion of DNA using restriction enzymes Sfi I or Not I.

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  • Analysis of digested DNA by pulsed-field gel electrophoresis (PFGE).

    • Main Results:

    • Eight distinct PFGE patterns were identified among the 56 strains.
    • Most CT-gene-positive epidemic strains from India, Bangladesh, and Thailand exhibited similar PFGE patterns.
    • CT-positive strains from India and Nepal showed slight variations, while CT-negative strains from Argentina, Sri Lanka, and Bangladesh displayed significant genetic differences from each other and from epidemic strains.
    • A V. cholerae O1 E1 Tor strain from India shared similarities with epidemic V. cholerae O139 strains.

    Conclusions:

    • PFGE analysis highlights genetic heterogeneity within Vibrio cholerae O139.
    • Epidemic strains demonstrate a degree of genetic relatedness, suggesting common origins or successful clonal expansion.
    • Non-epidemic and CT-negative strains possess distinct genetic profiles, indicating separate evolutionary trajectories or different ecological niches.