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Fixed bin frequency distributions for the VNTR locus D5S110 in general United States reference databases
1Forensic Science Research and Training Center, FBI Academy, Quantico, VA, USA.
Journal of Forensic Sciences
|March 1, 1995
Summary
DNA forensic analysis is enhanced by new data on the D5S110 locus. Fixed bin frequencies were determined for diverse US populations, aiding in paternity testing and profile frequency estimation.
Area of Science:
- Forensic Genetics
- Population Genetics
Background:
- Variable Number Tandem Repeat (VNTR) loci are crucial for DNA profiling.
- Accurate population frequency data is essential for forensic and paternity testing.
- The D5S110 locus is a commonly used VNTR marker.
Purpose of the Study:
- To determine fixed bin frequencies for the D5S110 locus in diverse United States populations.
- To assess the genetic behavior of the D5S110 locus within these populations.
- To provide data for forensic and paternity applications.
Main Methods:
- Restriction Fragment Length Polymorphism (RFLP) analysis was employed.
- Genomic DNA was digested using HAE III.
- Allele frequencies were calculated for African Americans, Caucasians, Southeastern Hispanics, and Southwestern Hispanics.
Main Results:
- The D5S110 locus demonstrated adherence to Hardy-Weinberg expectations across all four populations.
- No significant association was found between D5S110 alleles and other commonly used VNTR loci.
- Fixed bin frequencies for D5S110 were established for the studied demographic groups.
Conclusions:
- The determined D5S110 allele frequencies are reliable for use in forensic casework.
- This data supports the accurate estimation of DNA profile frequencies in general US populations.
- The D5S110 locus is genetically stable and suitable for population-based forensic identification.