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Systematic toxicological analysis using HPLC/DAD

A Tracqui1, P Kintz, P Mangin

  • 1Institut de Médecine Légale, Faculté de Médecine de Strasbourg, France.

Journal of Forensic Sciences
|March 1, 1995
PubMed
Summary
This summary is machine-generated.

A new high-performance liquid chromatography method with diode-array detection (HPLC/DAD) enables rapid toxicological analysis of blood and plasma. This technique efficiently screens for 311 substances, offering a simple, specific, and cost-effective solution for drug identification.

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Area of Science:

  • Analytical Chemistry
  • Forensic Toxicology

Background:

  • Toxicological analysis of biological samples is crucial for identifying substances.
  • Existing methods may lack efficiency or specificity for comprehensive screening.
  • High-performance liquid chromatography with diode-array detection (HPLC/DAD) offers potential for improved drug analysis.

Purpose of the Study:

  • To develop and validate a systematic toxicological analysis method for human blood or plasma using HPLC/DAD.
  • To establish a rapid, specific, and cost-effective approach for identifying a wide range of pharmaceuticals, toxicants, and drugs of abuse.

Main Methods:

  • A single-step liquid/liquid extraction procedure was employed.
  • Isocratic elution was performed on a NovaPak C18 column at 30°C.
  • On-line UV spectral recording (200-400 nm) and automated library comparison were utilized for identification.

Main Results:

  • The method successfully analyzed human blood or plasma samples.
  • A library of 311 reference compounds (pharmaceuticals, toxicants, drugs of abuse) was established for automated identification.
  • The chromatographic run time was 20 minutes, with a flow rate of 0.8 mL/min.

Conclusions:

  • The developed HPLC/DAD method is simple, rapid, inexpensive, and highly specific for toxicological analysis.
  • This technique facilitates systematic screening and identification of a broad spectrum of substances in biological samples.
  • The study highlights the utility and limitations of HPLC/DAD in drug screening applications.