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Thyroid function testing evaluated on three immunoassay systems

R H Christenson1, S H Duh, D E Clarisse

  • 1Department of Pathology, University of Maryland, School of Medicine, Baltimore, USA.

Journal of Clinical Laboratory Analysis
|January 1, 1995
PubMed
Summary
This summary is machine-generated.

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The Abbott IMx system demonstrated superior performance in thyroid function testing compared to ACS 180 and Stratus II. It showed the best diagnostic accuracy for free thyroxine (FT4) and thyroid-stimulating hormone (TSH) assays.

Area of Science:

  • Clinical Chemistry
  • Immunoassay Technology
  • Thyroid Diagnostics

Background:

  • Thyroid function tests are crucial for diagnosing thyroid disorders.
  • Evaluating the performance of automated immunoassay systems is essential for clinical laboratories.
  • Comparison of different analytical platforms ensures reliable patient results.

Purpose of the Study:

  • To compare the analytical performance and diagnostic accuracy of three automated immunoassay systems: ACS 180, Abbott IMx, and Stratus II.
  • To assess the reliability of thyroid-stimulating hormone (TSH), total thyroxine (TT4), and free T4 (FT4) assays across these platforms.
  • To determine the best performing system for routine thyroid function testing.

Main Methods:

  • Assessed TSH, TT4, and FT4 assays using ACS 180, Abbott IMx, and Stratus II systems.

Related Experiment Videos

  • Evaluated TSH assay sensitivity, imprecision, and accuracy.
  • Determined TT4 and FT4 imprecision and accuracy, including method agreement.
  • Compared laboratory diagnoses derived from FT4 and calculated FT4 Index results against routine and altered protein specimens.

    • Main Results:

    • Abbott IMx and ACS 180 were identified as second-generation TSH assays; Stratus II showed intermediate performance.
    • TSH imprecision ranged from 3-10% with adequate accuracy for all systems.
    • FT4 imprecision varied: Stratus II (20%), IMx (<5%), and ACS 180 (6%).
    • Abbott IMx exhibited the highest diagnostic accuracy, with 0% discordant results in altered protein groups, compared to ACS 180 (3%) and Stratus II (9%).

    Conclusions:

    • The Abbott IMx system demonstrated the best overall performance among the evaluated platforms.
    • IMx offered superior diagnostic accuracy for FT4 testing, particularly in challenging patient samples.
    • The findings support the Abbott IMx as a reliable system for comprehensive thyroid function assessment.