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Related Experiment Videos

Glucose-containing oligosaccharides in normal human urine

M Tokunaga, M Endo, Z Yosizawa

    The Tohoku Journal of Experimental Medicine
    |January 1, 1979
    PubMed
    Summary

    Healthy individuals consistently excrete neutral oligosaccharides in urine, with a uniform excretion pattern observed across different ages and sexes. Glucosylgalactose was identified as a key component in these urinary saccharides.

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    Area of Science:

    • Biochemistry
    • Human Physiology
    • Analytical Chemistry

    Background:

    • Urine composition analysis is crucial for understanding human metabolic processes.
    • Neutral saccharides in urine are often overlooked but may hold diagnostic significance.

    Purpose of the Study:

    • To characterize neutral oligosaccharides present in normal human urine.
    • To determine if urinary neutral saccharide excretion patterns vary with age or sex.

    Main Methods:

    • Urine samples were treated with cetylpyridinium chloride (CPC) and sequentially passed through ion-exchange columns (Dowex 50 and Dowex 1).
    • Gel filtration chromatography using Sephadex G-10 separated neutral saccharides into subfractions.
    • Paper chromatography was employed to identify and characterize specific saccharide components, including Component A.

    Main Results:

    • A consistent pattern of neutral oligosaccharide excretion was observed in healthy individuals.
    • Excretion patterns remained similar irrespective of the individuals' age or sex.
    • Component A, isolated from a specific subfraction, was identified as glucosylgalactose.

    Conclusions:

    • Normal human urine contains neutral oligosaccharides excreted in a consistent pattern.
    • This consistent pattern suggests a stable metabolic pathway for these saccharides.
    • Glucosylgalactose is a identified neutral oligosaccharide in human urine.

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