[Isolation of Helicobacter pylori from gastric mucosa depending on the growth medium used]

The usefulness of selective media in increasing the percentage of isolated Helicobacter pylori as well as the occurrence of H. pylori infection in upper gastro-intestinal tract disorders were evaluated. It was shown that the frequency of H. pylori isolation was significantly higher when selective media (Columbia agar with supplementation according to Skirrow-Campylobacter Selective Supplement SR 69 and Columbia agar with Helicobacter pylori Selective Supplement SR 147,Oxoid) were used instead of non-selective media (Columbia agar). Upper gastrointestinal endoscopy performed in 125 patients demonstrated that H. pylori infection was rather frequent (50%) and dependent on the type of pathology. It was almost always accompanied by histological features of chronic inflammation of mucosa. In patients with gastric bile reflux the frequency of H. pylori infection was about 19%.