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Inflammatogenic properties of nucleus pulposus
K Olmarker1, J Blomquist, J Strömberg
1Department of Orthopaedics, Sahlgren Hospital, Sweden.
Spine
|March 15, 1995
Summary
Nucleus pulposus demonstrated inflammatogenic properties, attracting significantly more leukocytes and increasing vascular permeability in experimental models. Further research is needed to determine if these reactions are from nucleus pulposus components directly or from secondary tissue responses.
Area of Science:
- Biomedical Science
- Inflammation Research
- Spinal Pathophysiology
Background:
- Inflammatory mechanisms are implicated in sciatica pathogenesis from herniated nucleus pulposus.
- Clinical observations and experimental studies suggest a role for inflammation.
Purpose of the Study:
- To assess the inflammatogenic properties of nucleus pulposus.
- To utilize established experimental models for screening inflammatogenic substances.
Main Methods:
- Two experimental models were employed: subcutaneous implantation of nucleus pulposus in pigs and vital microscopy of hamster cheek pouches.
- Leukocyte counts, microvascular reactions, and macromolecular leakage were evaluated.
Main Results:
- Nucleus pulposus significantly attracted more leukocytes compared to fat controls.
- Injections of nucleus pulposus induced thrombosis and significant macromolecular leakage.
- Control injections of fat or vehicle showed minimal changes.
Conclusions:
- Nucleus pulposus exhibits inflammatogenic properties, evidenced by leukotaxis and increased vascular permeability.
- The precise source of these reactions (nucleus pulposus components vs. secondary tissue response) requires further investigation.