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Hyperthermia induces ultrastructural changes in mouse pial microvessels

M A Fahim1, F el-Sabban

  • 1Department of Physiology, Faculty of Medicine and Health Sciences, U.A.E. University, Al Ain, United Arab Emirates.

The Anatomical Record
|May 1, 1995
PubMed
Summary

Local hyperthermia causes significant damage to mouse pial microvessels, leading to platelet activation and endothelial cell changes. These ultrastructural alterations highlight early signs of vascular pathology following heat exposure.

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Area of Science:

  • Neuroscience
  • Vascular Biology
  • Cellular Biology

Background:

  • Local hyperthermia can induce spontaneous thrombosis in pial microvessels.
  • Cellular and subcellular changes underlying hyperthermia-induced thrombosis were previously unexplored.

Purpose of the Study:

  • To investigate the ultrastructural effects of regional hyperthermia (43°C) on mouse pial microvessels.
  • To identify cellular and subcellular changes contributing to thrombosis development.

Main Methods:

  • Utilized a cranial window with artificial cerebrospinal fluid delivery and heating for precise temperature control (43°C).
  • Maintained core body temperature at 37°C.
  • Employed bimodal fixation and standard electron microscopy for ultrastructural analysis.

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Main Results:

  • Control microvessels (37°C) showed no cellular damage.
  • Hyperthermic microvessels (43°C) exhibited platelet activation (varied forms, degranulation) and aggregation near the endothelium.
  • Significant endothelial changes observed, including denudation, vacuole formation, and membrane rupture.

Conclusions:

  • Local hyperthermia causes substantial damage to pial microvasculature.
  • Endothelial cell changes are early indicators of vascular pathology induced by hyperthermia.