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Overexpression in Escherichia coli of 12 vitamin B12 biosynthetic enzymes
C A Roessner1, J B Spencer, S Ozaki
1Center for Biological NMR, Department of Chemistry, Texas A&M University, College Station 77843-3255, USA.
Protein Expression and Purification
|April 1, 1995
Abstract:
The first 12 enzymes involved in the biosynthesis of vitamin B12 from its five-carbon precursor, aminolevulinic acid, have been overexpressed in recombinant strains of Escherichia coli. The activity of each enzyme has been demonstrated by the biosynthesis of hydrogenobyrinic acid from aminolevulinic acid.