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The mRNA-binding protein COLBP is glutamate dehydrogenase
T Preiss1, A E Sang, Z M Chrzanowska-Lightowlers
1Division of Clinical Neuroscience, University of Newcastle upon Tyne, The Medical School, UK.
Abstract:
Expression of the liver-type isopeptides of cytochrome c oxidase is regulated post-transcriptionally. An RNA-binding activity has been found in only those cells where the liver-type subunits are detected. This binding protein, termed COLBP, recognises sequences or structures within the 3'-untranslated regions of transcripts encoding these liver-type isopeptides and has been implicated in the modulation of mRNA expression. We now show by subcellular fractionation, immunocompetition, UV-crosslinking and shift-Western studies that the metabolic enzyme glutamate dehydrogenase, previously reported as being able to bind RNA, is the cytochrome c oxidase transcript-binding protein, COLBP.