Related Experiment Video
Updated: Aug 13, 2026

Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency
Published on: June 16, 2011
Small differences in electrophoretic mobility among circularly permuted sequence isomers of duck hepatitis B virus
1Department of Microbiology and Immunology, School of Medicine, University of North Carolina at Chapel Hill 27599-7290, USA.
Abstract:
Two linear minus-strand viral DNAs, disparate in size by 10 nucleotides, were isolated from duck hepatitis B virus infected tissues and observed to migrate differently in nondenaturing agarose gels. We examined this phenomenon using both synthetic and cloned viral DNAs and discovered that distinct, circularly permuted, linear isomers of single-stranded DNA could have slightly different electrophoretic mobilities under nondenaturing conditions. This finding reveals a novel feature for consideration in assessing the conformations of native or renatured single-stranded nucleic acids. The study also suggests that the virion-derived minus-strand DNAs of the avihepadnaviridae may necessarily possess a minimal secondary structure.
Related Concept Videos
Viral Mutations
Size and Structure of Viral Genomes
Viruses with RNA Genomes
Hepatitis

