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Non-reciprocal regulation of Rhodobacter capsulatus and Rhodobacter sphaeroides recA genes expression
A R Fernandez de Henestrosa1, E Rivera, J Barbé
1Department of Genetics and Microbiology, Faculty of Sciences, Autonomous University of Barcelona, Bellaterra, Spain.
Abstract:
The Rhodobacter capsulatus recA gene has been isolated and sequenced. Its deduced amino acid sequence showed the closest identity with the Rhodobacter sphaeroides RecA protein (91% identity). However, the promoter regions of both R. capsulatus and R. sphaeroides recA genes are only 64% similar. An Escherichia coli-like LexA binding site was not present in the upstream region of the R. capsulatus recA gene. Nevertheless, the R. capsulatus recA gene is inducible by DNA damage in both hetero- and phototrophically growing conditions. The R. capsulatus recA gene is poorly induced when inserted into the chromosome of R. sphaeroides, indicating that the recA gene of both bacteria possess different control sequences despite their phylogenetically close relationship.