PCR-directed preparation and single-step purification of highly active histidine-tagged restriction endonuclease
1Institut für Mikrobiologie und Molekularbiologie, Justus-Liebig-Universität Giessen, Germany.
Gene
|May 19, 1995
Abstract:
The polymerase chain reaction was used to produce His6 fusion proteins via deletion of an intervening piece of DNA. The generally applicable method was performed using a standard primer with the advantage that the fusion does not produce additional amino acids. In a single-step purification highly purified, enzymatically active restriction endonuclease was obtained.


