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Mutational analysis of conserved amino-acid motifs in EcoKI adenine methyltransferase
D T Dryden1, D F Willcock, N E Murray
1Institute of Cell and Molecular Biology, University of Edinburgh, UK.
Gene
|May 19, 1995
Abstract:
The EcoKI methyltransferase (M.EcoKI, MTase) contains the amino acid (aa) sequences AAGTA and NPPF believed to represent the two sequences that are strongly conserved in adenine MTases [Klimasauskas et al., Nucleic Acids Res. 17 (1989) 9823-9831]. We have analysed a mutation in the first sequence that abolishes cofactor binding and enzyme activity, and mutations in the second sequence that reduce or abolish activity without affecting cofactor and DNA binding.