Purification and characterization of the restriction endonuclease AvcI from Actinomyces cristalomycini

The restriction endonuclease AvcI, an isoschizomer of Sau96I [Sussenbach et al., Nucleic Acids Res. 5 (1978) 1153-1163], was purified from Actinomyces cristalomycini. AvcI recognizes a 5-bp palindromic sequence, 5'-G decreases GNCC and cleaves it after the first G residue producing a 3-nucleotide 5'-overhang.