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Related Experiment Videos

A transposon-like sequence adjacent to the AccI restriction-modification operon

S Brassard1, H Paquet, P H Roy

  • 1Département de Biochimie, Faculté des Sciences et de Génie, Université Laval, Ste-Foy, Québec, Canada.

Gene
|May 19, 1995
PubMed
Summary
This summary is machine-generated.

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Researchers sequenced Weeksella zoohelcum

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • The accIRM genes were previously identified in Acinetobacter calcoaceticus.
  • A strain originally identified as A. calcoaceticus was reclassified as Weeksella zoohelcum.

Purpose of the Study:

  • To clone and sequence the accIRM genes from Weeksella zoohelcum.
  • To characterize adjacent genes and regulatory elements within the W. zoohelcum genome.

Main Methods:

  • DNA cloning and sequencing techniques were employed.
  • Bioinformatic analysis was used to identify genes and predict their functions.
  • Sequence comparison with previously published data was performed.

Main Results:

  • The accIRM gene sequence from W. zoohelcum was determined, revealing a three-nucleotide addition in the accIM gene compared to prior reports.

Related Experiment Videos

  • Two novel genes, convergently transcribed with the accIRM operon, were identified.
  • One gene encodes a protein homologous to phage integrase family recombinases, potentially acting as a transposon resolvase.
  • The second gene encodes a putative transposase (Tnp).
  • Distinct Rho-dependent and Rho-independent terminators were predicted for the two operons.

    • Conclusions:

    • The findings provide a detailed genomic characterization of the accIRM operon and associated genes in W. zoohelcum.
    • The identified recombinase and transposase suggest the presence of a mobile genetic element.
    • The distinct G+C content and predicted terminators support the hypothesis of a mobile element boundary.