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Overproduction of His-tagged EcoRII restriction endonuclease and terminally deleted mutant proteins
D Kupper1, M Reuter, D H Krüger
1Institute of Medical Virology, Humboldt University School of Medicine (Charité), Berlin, Germany.
Gene
|May 19, 1995
Abstract:
EcoRII was the first restriction endonuclease (ENase) reported requiring the cooperative interaction with at least two DNA sites for activity. Using two different expression systems the enzyme could be purified and its special substrate requirements were further analyzed. At the present state of knowledge we suggest a model of simultaneous binding of two DNA sites to one dimeric enzyme molecule (see Krüger et al., FEMS Microbiol. Rev. (1995) in press).