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Isolation of cDNA clones encoding the mouse protein V-1
D Pennica1, K J Shaw, S M Luoh
1Department of Molecular Biology, Genentech, Inc., South San Francisco, CA 94080, USA.
Gene
|June 9, 1995
Summary
Researchers identified mouse V-1, a brain protein identical to rat V-1. Despite sharing similarities with myotrophin, mouse V-1 did not induce cardiac myocyte hypertrophy in experiments.
Area of Science:
- Molecular Biology
- Developmental Neuroscience
- Cardiology
Background:
- V-1 is a developmentally regulated brain protein.
- Myotrophin is a protein known to induce cardiac myocyte hypertrophy.
- Previous studies suggest potential functional links between V-1 and myotrophin.
Purpose of the Study:
- To isolate and characterize mouse V-1.
- To investigate the relationship between mouse V-1 and myotrophin.
- To determine if mouse V-1 can induce cardiac myocyte hypertrophy.
Main Methods:
- Used rat V-1 amino acid sequence to screen a mouse embryoid body cDNA library.
- Sequenced the isolated mouse V-1 gene.
- Produced recombinant mouse V-1 in Escherichia coli.
- Assayed the ability of recombinant mouse V-1 to induce cardiac myocyte hypertrophy.
Main Results:
- Identified mouse V-1, an 118 amino acid protein.
- Mouse V-1 shares identical amino acid sequences with rat V-1.
- Mouse V-1 exhibited significant sequence similarity to myotrophin, including a 23/24 amino acid match in a tryptic peptide.
- Recombinant mouse V-1 failed to induce cardiac myocyte hypertrophy.
Conclusions:
- Mouse V-1 is a conserved protein across species with identical sequences to rat V-1.
- Despite sequence homology, mouse V-1 does not possess the hypertrophic activity attributed to myotrophin.
- The functional role of V-1 in cardiac myocyte hypertrophy remains unconfirmed.