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Lgn1, a gene that determines susceptibility to Legionella pneumophila, maps to mouse chromosome 13
W F Dietrich1, D M Damron, R R Isberg
1Whitehead Institute/MIT Genome Center, Cambridge, Massachusetts 02139, USA.
Abstract:
The intracellular pathogen Legionella pneumophila is unable to replicate in macrophages derived from most inbred mouse strains. Here, we report the mapping of a gene, called Lgn1, that determines whether mouse macrophages are permissive for the intracellular replication of L. pneumophila. Although Lgn1 has been previously reported to map to mouse chromosome 15, we show here that it actually maps to chromosome 13, between D13Mit128 and D13Mit70. In the absence of any regional candidates for Lgn1, this map position will facilitate positional cloning attempts directed at this gene.
Insights
A newly mapped gene, Lgn1, controls Legionella pneumophila replication in mouse macrophages. This finding refines the gene
Area of Science:
- Immunology
- Genetics
- Microbiology
Background:
- Legionella pneumophila is an intracellular pathogen that cannot replicate in macrophages from most mouse strains.
- The genetic basis for this permissiveness is not fully understood.
Purpose of the Study:
- To map the Lgn1 gene, which determines macrophage permissiveness to L. pneumophila intracellular replication.
- To identify the precise chromosomal location of Lgn1.
Main Methods:
- Genetic mapping of the Lgn1 gene in mouse populations.
- Analysis of previously reported mapping data.
Main Results:
- The Lgn1 gene was mapped to mouse chromosome 13, between markers D13Mit128 and D13Mit70.
- This contradicts previous reports placing Lgn1 on chromosome 15.
Conclusions:
- The refined map position of Lgn1 on chromosome 13 provides a critical resource for positional cloning.
- This will aid in the identification of the specific gene responsible for L. pneumophila replication control in macrophages.