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Cell adhesion and spreading factor: chemical modification studies

F Grinnell, D Minter

    Biochimica Et Biophysica Acta
    |January 5, 1979
    PubMed
    Summary
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    Chemical modifications reveal that protein components, not carbohydrates, are crucial for fetal calf serum factor

    Area of Science:

    • Cell biology
    • Biochemistry

    Background:

    • Fetal calf serum (FCS) contains factors promoting cell adhesion and spreading.
    • Understanding the molecular basis of this factor's activity is essential for cell culture applications.

    Purpose of the Study:

    • To identify the specific molecular components of the FCS factor responsible for promoting cell adhesion and spreading.
    • To elucidate the role of protein and carbohydrate moieties in the factor's biological activity.

    Main Methods:

    • Purification of the FCS factor.
    • Chemical modifications targeting carbohydrate and protein portions.
    • Assays for cell adhesion and spreading on tissue culture substrata.
    • Control experiments to assess non-specific adsorption.

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    Main Results:

    • Modification of carbohydrate portions (glycosidic enzymes, periodate oxidation) did not affect factor activity.
    • Modification of protein portions (-COOH, tyrosine, tryptophan residues) significantly inhibited activity.
    • Modification of other protein residues (-SH, -NH2, methionine) had no impact on activity.

    Conclusions:

    • Protein components, specifically certain amino acid residues, are critical for the cell adhesion and spreading activity of the FCS factor.
    • Carbohydrate portions are not essential for this specific biological function.