Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Two recA genes in Myxococcus xanthus

N Norioka1, M Y Hsu, S Inouye

  • 1Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA.

Journal of Bacteriology
|July 1, 1995
PubMed
Summary

Researchers identified two recA genes, recA1 and recA2, in Myxococcus xanthus. Both genes, recA1 and recA2, were found to complement UV sensitivity in E. coli, indicating functional roles in DNA repair.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

GHKL, an emergent ATPase/kinase superfamily.

Trends in biochemical sciences·2000
Same author

Antisense RNA and DNA in Escherichia coli.

Methods in enzymology·1999
Same author

Dicarboxylic acids as markers of fatty acid peroxidation in diabetes.

Atherosclerosis·1999
Same author

Modulation of chemokine expression during ischemia/reperfusion in transgenic mice overproducing human glutathione peroxidases.

Journal of immunology (Baltimore, Md. : 1950)·1999
Same author

Histidine kinases: diversity of domain organization.

Molecular microbiology·1999
Same author

A retroelement in Vibrio cholerae.

Molecular microbiology·1999

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • The RecA protein is crucial for DNA repair and recombination in bacteria.
  • Understanding RecA homologs in diverse bacterial species like Myxococcus xanthus provides insights into conserved and divergent DNA repair mechanisms.

Purpose of the Study:

  • To clone and characterize the two recA genes (recA1 and recA2) in Myxococcus xanthus.
  • To investigate the expression patterns and functional complementation of these genes.

Main Methods:

  • Cloning of recA genes using the Escherichia coli recA gene as a probe.
  • DNA sequencing and deduced amino acid sequence analysis.
  • Northern blot analysis for gene expression.
  • Complementation assays in an E. coli recA mutant strain.

Main Results:

  • Two homologous recA genes, recA1 and recA2, were identified and sequenced in Myxococcus xanthus.
  • RecA1 and RecA2 share significant amino acid identity with each other and with E. coli RecA.
  • recA2 expression is detected in both vegetative and developmental stages and is induced by nalidixic acid.
  • A recA1 disruption mutant was isolated, but recA2 disruption attempts failed, suggesting potential functional redundancy or essentiality.
  • Both recA1 and recA2 successfully complemented the UV sensitivity of an E. coli recA strain.

Conclusions:

  • Myxococcus xanthus possesses two functional recA genes, recA1 and recA2, involved in DNA repair.
  • The differential ease of isolating disruption mutants suggests distinct roles or regulation between recA1 and recA2.
  • These findings contribute to understanding the genetic basis of DNA repair in Myxococcus xanthus.

Related Experiment Videos