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Related Experiment Videos

Lewis X structure increases cell substratum adhesion in L cells

A Sudou1, M Ozawa, T Muramatsu

  • 1Department of Biochemistry, Nagoya University School of Medicine.

Journal of Biochemistry
|February 1, 1995
PubMed
Summary

The Le(x) antigen enhances cell adhesion by interacting with integrins. Fucosylation of basigin may be key to this integrin-mediated cell-substratum interaction.

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Area of Science:

  • * Cell biology
  • * Glycobiology
  • * Molecular biology

Background:

  • * The Le(x) antigen is a carbohydrate structure involved in cell recognition.
  • * Integrins are crucial cell surface receptors mediating cell adhesion.
  • * Understanding Le(x) antigen's role in cell adhesion requires investigating its molecular interactions.

Purpose of the Study:

  • * To investigate the role of the Le(x) antigen in cell adhesion.
  • * To identify the molecular mechanisms underlying Le(x)-mediated cell adhesion.
  • * To explore the potential involvement of integrins and specific proteins in this process.

Main Methods:

  • * Transfection of L cells with alpha-1,3-fucosyltransferase and alpha-1,3/4-fucosyltransferase cDNAs.
  • * Measurement of cell substratum adhesion in transfected cells.
  • * Inhibition studies using cycloheximide, anti-integrin antiserum, RGD peptide, and EGTA.
  • * Western blotting to identify Le(x) antigen carriers.

Main Results:

  • * Transfected cells expressing Le(x) antigen showed significantly increased cell substratum adhesion.
  • * Increased adhesion was inhibited by cycloheximide, anti-integrin antiserum, RGD peptide, and EGTA.
  • * Western blotting identified an 85 kDa protein and a 50-60 kDa protein (likely basigin) carrying the Le(x) antigen.
  • * Basigin, an integrin-associated protein, is hypothesized to be fucosylated.

Conclusions:

  • * The Le(x) structure promotes cell adhesion to cell-secreted substratum-bound material.
  • * Integrin-mediated mechanisms are responsible for the increased cell adhesion.
  • * Fucosylation of basigin may enhance integrin-mediated cell substratum adhesion.

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