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Transchromosomally derived Ig heavy chains

K L Knight1, M Kingzette, M A Crane

  • 1Department of Microbiology and Immunology, Stritch School of Medicine, Loyola University Chicago, Maywood, IL 60153, USA.

Journal of Immunology (Baltimore, Md. : 1950)
|July 15, 1995
PubMed
Summary
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In rabbits, immunoglobulin heavy chain genes can associate across chromosomes during the immune response. This trans-chromosomal association occurs during B cell isotype switching, not VDJ gene rearrangement.

Area of Science:

  • Immunology
  • Molecular Biology
  • Genetics

Background:

  • Activated B cells switch from expressing IgM and IgD to other isotypes like IgA.
  • Isotype switching involves gene rearrangement, typically within the same chromosome (in cis).
  • Previous studies identified IgA heavy chains in rabbits with gene origins from different chromosomes (in trans).

Purpose of the Study:

  • To determine if trans-chromosomal association of VH and C alpha genes occurs during VDJ gene rearrangement or isotype switching.
  • To elucidate the chromosomal origin of VH, JH, and C alpha regions in trans IgA heavy chains.

Main Methods:

  • Cloning of rabbit cDNA encoding trans IgA heavy chains.
  • Analysis of nucleotide polymorphism in JH regions.
  • Analysis of VH allotype.

Related Experiment Videos

  • DNA polymorphism analysis of JH and C alpha regions.

    • Main Results:

    • VH and JH gene rearrangements occurred in cis, indicating VDJ recombination happens on the same chromosome.
    • VDJ and C alpha genes encoding trans IgA molecules were found on different parental chromosomes.
    • Trans-chromosomal association of VH and CH genes was confirmed.

    Conclusions:

    • Trans-chromosomal association of VH and CH genes occurs during isotype switching, not VDJ gene rearrangement.
    • This trans-association is part of a normal immune response in rabbits.