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Related Experiment Videos

Normal ranges for immunochemiluminometric gonadotropin assays

E K Neely1, R L Hintz, D M Wilson

  • 1Department of Pediatrics, Stanford University, CA 94305, USA.

The Journal of Pediatrics
|July 1, 1995
PubMed
Summary
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New immunochemiluminometric assays (ICMA) provide sensitive measurement of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) in children. These assays accurately establish normative data for spontaneous and GnRH-stimulated gonadotropin levels, aiding pubertal status assessment.

Area of Science:

  • Pediatric endocrinology
  • Reproductive biology
  • Clinical chemistry

Background:

  • Accurate measurement of gonadotropins (LH and FSH) is crucial for assessing pubertal development in children and adolescents.
  • Traditional assays have limitations in sensitivity, particularly for low prepubertal levels.
  • Newer immunochemiluminometric assays (ICMA) offer improved sensitivity for hormone measurement.

Purpose of the Study:

  • To establish normative data for spontaneous and GnRH-stimulated serum LH and FSH levels in children and adolescents using new ICMA.
  • To evaluate the performance of ICMA compared to other assay methods (immunofluorometric assay and radioimmunoassay).

Main Methods:

  • Collected random serum samples from 375 normal children (0.1–17.7 years).
  • Performed GnRH stimulation tests in 41 normal subjects (4.8–18 years).

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  • Calculated normal ranges by age and Tanner stage, comparing ICMA with other assays.

    • Main Results:

    • Gonadotropin levels showed transient infant elevation, low prepubertal levels, and increased pubertal values.
    • Spontaneous LH was higher in male infants; FSH was higher in female infants.
    • ICMA demonstrated high correlation with immunofluorometric assay for LH, but diverged from radioimmunoassay at low concentrations.

    Conclusions:

    • ICMA offers significantly enhanced sensitivity for LH measurement compared to older methods.
    • ICMA accurately measures low prepubertal LH levels, serving as a biochemical discriminator of pubertal status.
    • A GnRH-stimulated LH level >5 IU/L by ICMA suggests maturing gonadotropin secretion.