Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Programmed cell death during mammary gland involution

R Strange1, R R Friis, L T Bemis

  • 1Division of Laboratory Research, AMC Cancer Research Center, Lakewood, Colorado 80214, USA.

Methods in Cell Biology
|January 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Identification of heat shock protein 10 within the equine embryo, endometrium, and maternal peripheral blood mononuclear cells.

Theriogenology·2014
Same author

Establishment and characterization of a new and stable collagen-binding assay for the assessment of von Willebrand factor activity.

International journal of laboratory hematology·2012
Same author

Electroporation-mediated gene transfer directly to the swine heart.

Gene therapy·2012
Same author

Pathophysiology of beta2-glycoprotein I in antiphospholipid syndrome.

Lupus·2010
Same author

Prepubertal physical activity up-regulates estrogen receptor beta, BRCA1 and p53 mRNA expression in the rat mammary gland.

Breast cancer research and treatment·2008
Same author

EGFR regulation by microRNA in lung cancer: correlation with clinical response and survival to gefitinib and EGFR expression in cell lines.

Annals of oncology : official journal of the European Society for Medical Oncology·2008
Same journal

Quantification of cell viability by automated analysis of live cell imaging.

Methods in cell biology·2026
Same journal

Flow cytometry evaluation of cytotoxicity exerted by effector immune cells against tumor cells.

Methods in cell biology·2026
Same journal

Time-lapse confocal laser scanning microscopy analysis of FOOD formation.

Methods in cell biology·2026
Same journal

Screening and identification of protein-protein interaction using proximity labeling.

Methods in cell biology·2026
Same journal

Quantitative high-content profiling of mitochondrial morphology with automated statistical analysis and integrated data visualization.

Methods in cell biology·2026
Same journal

Super-resolution imaging of cell death in Drosophila tissues via expansion and pan-expansion microscopy.

Methods in cell biology·2026
See all related articles

Mammary gland involution involves complex gene expression and protein interactions leading to cell death and renewal. Epithelial-mesenchymal interactions are crucial for maintaining differentiated function and regulating apoptosis during this process.

Area of Science:

  • Cell Biology
  • Developmental Biology
  • Cancer Research

Background:

  • Mammary gland involution is a complex process involving epithelial cell death and tissue remodeling for subsequent lactation cycles.
  • Understanding the regulators and executors of apoptotic cell death during involution is challenging due to the intricate cascade of gene expression and protein interactions.
  • The relationship between mammary epithelium and its associated mesenchyme is critical for maintaining differentiated function, as evidenced by remodeling enzyme expression during involution.

Purpose of the Study:

  • To explore the role of epithelial-mesenchymal interactions in mammary gland involution and apoptotic cell death.
  • To investigate how extracellular matrix components influence the survival of secretory mammary epithelium.
  • To understand the implications of altered epithelial-mesenchymal interactions on mammary-specific gene expression and breast neoplasia prevention.

Related Experiment Videos

Main Methods:

  • Review of existing literature on mammary gland involution, gene expression, and protein interactions.
  • Analysis of studies investigating the role of extracellular matrix and mesenchymal factors in epithelial cell survival.
  • Examination of research on stimuli that alter differentiated function and induce apoptotic cell death in mammary epithelium.

Main Results:

  • Components of the extracellular matrix may act as survival factors or reservoirs for matrix-bound growth factors essential for secretory epithelium survival.
  • Perturbation of the epithelial-mesenchymal interaction significantly alters mammary-specific differentiation gene expression, including milk protein production.
  • Alterations in epithelial-mesenchymal interactions during involution can contribute to epithelial cell death, with potential implications for breast cancer control.

Conclusions:

  • Epithelial-mesenchymal interactions are integral to mammary gland involution and play a significant role in mediating or modulating epithelial cell death.
  • The interplay between the mammary epithelium and mesenchyme is vital for differentiated function and survival, and its disruption impacts involution.
  • Further definition of these interactions is necessary and holds potential for applications in the prevention and control of breast neoplasia.