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Serotonin activates phospholipase D in rat mesangial cells
D Kurscheid-Reich1, D C Throckmorton, H Rasmussen
1Department of Internal Medicine, Yale School of Medicine, New Haven, Connecticut 06510, USA.
The American Journal of Physiology
|June 1, 1995
Summary
Serotonin activates phospholipase D (PLD) in rat mesangial cells, impacting cell proliferation. This study reveals PLD
Area of Science:
- Cell Biology
- Biochemistry
- Physiology
Background:
- Serotonin (5-hydroxytryptamine, 5-HT) is a key neurotransmitter.
- Phospholipase D (PLD) is an enzyme involved in cell signaling.
- The role of serotonin in regulating PLD activity in mesangial cells is not well understood.
Purpose of the Study:
- To investigate the hypothesis that serotonin activates phospholipase D (PLD) in cultured rat mesangial cells.
- To elucidate the role of PLD in serotonin-mediated mesangial cell proliferation.
Main Methods:
- Measurement of PLD activity by quantifying phosphatidylethanol (PET) formation in the presence of ethanol.
- Assessment of phosphatidic acid and 1,2-diacylglycerol (DAG) levels.
- Evaluation of the effect of exogenous PLD on mesangial cell proliferation.
Main Results:
- Serotonin (5-HT) significantly stimulated PET production in a time-dependent manner.
- PET levels initially increased then decreased due to metabolism, indicating PLD activity.
- Inhibition of phosphatidic acid phosphohydrolase with propranolol altered phosphatidic acid and DAG levels.
- Exogenous PLD induced mesangial cell proliferation and increased phosphatidic acid, PET, and DAG.
Conclusions:
- Phosphatidic acid contributes to DAG formation in mesangial cells.
- PLD is implicated in serotonin-mediated mesangial cell proliferation.
- Serotonin signaling pathway involves PLD activation in rat mesangial cells.