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Isolation of SagI, a new HaeIII isoschizomer from Streptococcus agalactiae
1U. S. Department of Agriculture, Agricultural Research Service, Philadelphia, PA 19118, USA.
Applied Microbiology and Biotechnology
|May 1, 1995
Abstract:
A new HaeIII isoschizomer from Streptococcus agalactiae was isolated by a single-step purification method. The highly active restriction endonuclease, SagI, was free of nonspecific nuclease activity and was suitable for use in molecular biology procedures. The rapid isolation procedure may be applicable for the recovery of other restriction endonucleases from bacteria.